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Erythromycin ratiopharm 500 mg filmtabletten 250 for 7 days and 200 mg/kg for 7 days; in vivo n = 5, rats were challenged with 4 microg/ml L. rhusunensis at doses of 4 mg/kg (1×1010 CFU) and 30 CFU); oral administration (4.8 mg/kg) of 100 μg/kg L. rhusunensis extract led to a significantly higher dose-dependent inhibition of H3K9me1-mediated NF-κB activation than orally dosed vehicle control (p = 0.021 and p 0.037; Figure 3E); oral administration (28 mg/kg) of a high dose (>100 μg/kg) resulted in the accumulation of ROS liver and kidneys (Figure 3A) as compared to lower dose oral administration of L. rhusunensis extract at a lower dose (14 mg/kg) (Figure 3B), and L. rhusunensis induced oxidative stress in a dose-dependent manner (Table 1). TABLE 1 Table 1. Oral doses of L. rhusunensis extract, or extract with without 0.25 μg/ml of BSA or 0.5% NaCl, in experimental models of oxidative stress. TABLE 2 Table 2. In vitro bioeffects of L. rhusunensis oral doses (4, 24, 48, 96, and 200 μg/ml) L. rhusunensis oral doses (0.25 and 0.5 μg/ml of BSA or NaCl) on lipid peroxidation in human HepG2 hepatoma cultures. FIGURE 3 Figure 3. Cellular oxidative stress induced in liver and kidneys of L. rhusunensis-treated groups by oral administration (4 mg/kg) of L. rhusunensis extract. The number of CFU in indicated tissue, % of CFU L. rhusunensis extract in the respective subplaque, and mean (±SEM) changes in expression levels are depicted. *p<0.05. Discussion Oxidative stress is a major contributor to oxidative damage in human disease conditions, and an important factor responsible for the development of chronic disease (5). The beneficial effects of antioxidant compounds could be particular relevance to canada pharmacy online viagra the treatment of diseases due to oxidative stress, as antioxidant effects may delay the onset of oxidative stress-induced inflammation or apoptosis (6, 7). Although some of the reported therapeutic effects antioxidants have been attributed to free radical scavenging and/or other mechanisms, antioxidants may also act through antioxidant properties at the mitochondrial level (8). It is clear that oxidative stress often accompanied by inflammation. In this context, mitochondrial dysfunction has been implicated in the development of several diseases including hypertension, diabetes, cardiovascular disorder, cancer and neurodegeneration. In this situation, mitochondria play an important role as biological "factories" that generate free radicals and other reactive molecules in response to environmental insults, which lead the induction of genes involved in inflammation. this way, mitochondria-mediated oxidative stress can also impair the function of immune system and contribute to aging (3, 6). In our study, L. rhusunensis oral dose-dependently attenuated the generation of ROS in vitro and vivo by inhibiting mitochondrial uncoupling and NF-κB activation. In addition, L.

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